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. 2024 May 2;63(5):2301232. doi: 10.1183/13993003.01232-2023

FIGURE 3.

FIGURE 3

Deficiency of gasdermin B (GSDMB) impairs induction of interferon-stimulated genes (ISGs) by RNA mimic or RNA virus. a) Expression of RANTES and OASL in BEAS-2B cells with or without knockout (KO) of GSDMB after transfection of high molecular weight (HMW) polyinosinic-polycytidylic acid (poly(I:C)) for 6 h. b) RANTES protein levels in supernatants were measured in BEAS-2B cells transfected with HMW poly(I:C) for 6 h. c) Expression of RANTES and OASL in normal human bronchial epithelial cells (nHBEs) with or without GSDMB KO after transfection of HMW poly(I:C) for 6 h. d, e) Expression of RANTES in empty vector (EV) or GSDMB KO BEAS-2B cells infected with respiratory syncytial virus (RSV) line 19 (multiplicity of infection=1, 24 h) (d) or rhinovirus 16-A (RV-A16) (multiplicity of infection=1, 72 h) (e). f) Measurements of interferon regulatory factor 3 (IRF3) and TANK binding kinase 1 (TBK1) phosphorylation by immunoblotting in EV versus GSDMB-overexpressing cells. EV-transfected cells were used as controls. Mean±sems shown from at least three independent biological replicates. *: p<0.05; **: p<0.01 (two-way ANOVA).