FIGURE 6.

Human gasdermin B (hGSDMB) promotes respiratory syncytial virus (RSV)-induced mitochondrial antiviral-signalling protein (MAVS)-TANK binding kinase 1 (TBK1) signalling, inflammation and mucus production in mouse models. a) Representative haematoxylin and eosin (H&E) staining images of lung specimens from wild-type (WT), hGSDMB mice intranasally infected with 2×10⁴ pfu RSV line 19 (n=5) or PBS (n=5), respectively. Scale bars: 100 μm or 20 μm (inset). b) Peri-airway inflammation was scored based on the number of immune cells infiltrating surrounding airways as shown by H&E staining. c) Periodic acid–Schiff staining in WT and hGSDMB mice. Scale bars: 100 μm or 20 μm (inset). d) Western blotting of GSDMB and activated interferon regulatory factor 3 (IRF3) in lungs from mice in a. e) mRNA and protein levels of RANTES in murine lungs were measured by quantitative PCR and ELISA. f) Immunofluorescence staining of GSDMB (green) and RANTES (red) in murine lung slides. Nuclei were counterstained with DAPI (blue). Scale bars: 50 μm or 20 μm (inset). *: p<0.05; **: p<0.01 (two-way ANOVA).