Figure 2.

Suppression of lactate release upon endogenous HIF1α activation in breast cancer and glioblastoma cells

(A) Relative amount of extracellular lactate released by G55 control cells (n = 9) and G55 cells exposed to hypoxia (n = 3) or treated with DMOG (0.1 mM, n = 6 or 0.2 mM, n = 4).

(B) Relative amount of extracellular lactate released by MDA-MB-231 control cells or those treated with 0.5 mM DMOG (n = 6).

(C) Relative amount of extracellular lactate released by G55 control cells, HIF1α-silenced G55 cells, G55 cells treated with 0.2 mM DMOG, or HIF1α-silenced G55 cells treated with 0.2 mM DMOG (n = 6).

(D) Relative amount of extracellular lactate released by MDA-MB-231 control cells, HIF1α-silenced MDA-MB-231 cells, MDA-MB-231 cells treated with 0.5 mM DMOG, and HIF1α-silenced MDA-MB-231 cells treated with 0.5 mM DMOG (n = 5).

(E) Relative amount of extracellular lactate released by A549 control cells and A549 cells treated with 1 mM DMOG (n = 9), control murine embryonic fibroblasts (MEFs), and MEFs treated with 0.2 mM DMOG (n = 6).

In the bar graphs, the values represent the mean ± SEM, and statistical analysis was performed using a two-tailed unpaired t test when comparing 2 groups, with Welch’s correction when needed, or one-way or two-way ANOVA followed by Tukey’s post hoc test for (A) and (C) and for (D), respectively: ^∗p < 0.05 and ^∗∗∗p < 0.001 represent significant p values.