REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-HIF1α	BD Transduction Laboratories	Cat#610959; RRID: AB_398272
Mouse monoclonal anti-CAIX	Santa Cruz Biotechnology	Cat#sc-365900; RRID: AB_10846466
Monoclonal anti-β-actin-peroxidase	Sigma-Aldrich	Cat#A3854; RRID: AB_262011
Rabbit polyclonal anti-HIF-1α (C-Term)	Cayman Chemical	Cat#10006421; RRID: AB_409037
Rabbit polyclonal anti- PDK1	Enzo Life Sciences, Inc.	Cat#ADI-KAP-PK112; RRID: AB_10618932
Goat polyclonal anti-HSP 60	Santa Cruz Biotechnology	Cat#sc-1722; RRID: AB_2233354
Rabbit polyclonal anti-GAPDH	Abcam	Cat#ab9485; RRID: AB_307275
Rabbit polyclonal anti-GOT1	Proteintech	Cat#14886-1-AP; RRID AB_2113630
Rabbit polyclonal anti-GOT2	Sigma-Aldrich	Cat#HPA018139; RRID AB_1849903
Chemicals, peptides, and recombinant proteins
Doxycycline	Sigma-Aldrich	Cat#D9891
Dimethyloxalylglycine (DMOG)	Enzo Life Sciences, Inc.	Cat#BML-EI347
Metformin	Sigma-Aldrich	Cat#D150959
Sodium pyruvate	Lonza	Cat#BE13-115E
Sodium α-ketobutyrate (αKB)	Sigma-Aldrich	Cat#K0875
L-Glutamine-5-13 C	Cambridge Isotope Laboratories, Inc.	Cat#CLM-1166
Protease inhibitors	Sigma-Aldrich	Cat#P8340
Phosphatase inhibitors	Sigma-Aldrich	Cat#P5726
Pyridine	Sigma-Aldrich	Cat#270970
Methoxyamine hydrochloride	Sigma-Aldrich	Cat#226904
5-3H-Glucose	Perkin Elmer	Cat#NET531001MC
Dodecyltrimethylammonium bromide (DTAB)	Sigma-Aldrich	Cat#D8638
Critical commercial assays
L-Lactate Assay Kit (Colorimetric/Fluorometric)	Abcam	Cat#ab65330
DC protein Assay Kit	Bio-Rad	Cat#5000112
Pierce™ BCA Protein Assay Kit	Thermo Fisher Scientific	Cat#23225
NAD/NADH-Glo™ Assay Kit	Promega	Cat#G9071
Clarity Western ECL Substrate	Bio-Rad	Cat#1705061
Super-Signal West Femto Maximum Sensitivity Substrate	Thermo Fisher Scientific	Cat#34096
Experimental models: Cell lines
786-O	Provided by Dr. María J. Calzada	N/A
G55	Provided by Dr. Till Acker	N/A
MDA-MB 231	Provided by Dr. Massimiliano Mazzone	N/A
A549	Provided by Dr. Massimiliano Mazzone	N/A
Murine embryonic fibroblasts (MEFs)	This study	N/A
Oligonucleotides
Control siRNA	Santa Cruz Biotechnology	Cat#sc-37007
HIF1α siRNA	Santa Cruz Biotechnology	Cat#sc-35561
GAPDH siRNA	Santa Cruz Biotechnology	Cat#sc-35448
GOT1 siRNA	Dharmacon	Cat#J-011673
GOT2 siRNA	Dharmacon	Cat#J-011674
Recombinant DNA
pLVX-TetOne-Puro-Luc	Takara	Cat#631849
pLVX-TetOne-HIF1α	Meléndez-Rodríguez et al.18	N/A
pLVX-TetOne-HIF1α bHLH∗	Meléndez-Rodríguez et al.18	N/A
Other
Pyruvate-free Dulbecco’s high glucose modified Eagle’s Medium (DMEM)	Cytiva HyClone™	Cat#SH30022
Pyruvate- and glucose-free Dulbecco’s high glucose modified Eagle′s Medium (DMEM)	Gibco™	Cat#11966025
Pyruvate- and glutamine-free Dulbecco’s high glucose modified Eagle’s Medium (DMEM)	Gibco™	Cat#11960044
Pyruvate-, glutamine- and glucose-free Dulbecco’s high glucose modified Eagle’s Medium (DMEM)	Gibco™	Cat# A1443001
Lipofectamine 2000 Transfection Reagent	Invitrogen	Cat#11668019
Dialyzed fetal bovine serum (dFBS)	Sigma-Aldrich	Cat#F0392
Metabolic data from Human ccRCC samples	Hakimi et al.30	N/A
Mass spectrometry data	This study	EMBL-EBI MetaboLights: MTBLS8901 https://www.ebi.ac.uk/metabolights/MTBLS8901