| Antibodies |
|
| Living Colors® GFP Monoclonal Antibody |
Clontech |
Cat#632375, RRID:AB_2756343
|
| Anti-bZIP67 Purified Rabbit Immunoglobulin |
This manuscript |
N/A |
| Anti-ABI5 Purified Rabbit Immunoglobulin |
Albertos et al.2
|
N/A |
| Anti-PRX IIE |
Romero-Puertas et al.21
|
N/A |
| Anti-Actin clone 10-B3 Purified Mouse Immunoglobulin |
Sigma-Aldrich |
Cat#A0480, RRID:AB_476670
|
| ECL Rabbit HRP-linked |
GE Healthcare Life Sciences |
Cat#NA934, AB_772206 |
| ECL Mouse IgG, HRP-linked |
GE Healthcare Life Sciences |
Cat#NA931, RRID:AB_772210
|
| Monoclonal Anti-Biotin antibody produced in mouse clone BN-34 |
Sigma-Aldrich |
Cat#B7653, RRID:AB_258625
|
|
| Bacterial and virus strains |
|
| BL21(DE3) Competent E. coli |
NEB |
Cat#C2527I |
| One-Shot™ TOP10 E. coli
|
Invitrogen |
Cat#K240020 |
|
Agrobacterium tumefaciens strain C58C1 (pGV2260) |
Deblaere et al.54
|
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| Abscisic acid, ABA |
Sigma-Aldrich |
Cat#A1049 |
| 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, Carboxy-PTIO, cPTIO |
Thermofisher |
Cat#C7912 |
| 4,5-Diaminofluorescein diacetate solution, DAF-2DA |
Sigma-Aldrich |
Cat#D225 |
|
S-Nitrosoglutathione, GSNO |
Sigma-Aldrich |
Cat#N4148 |
| DL-Dithiothreitol, DTT |
Sigma-Aldrich |
Cat#D0632 |
| Bleach (4–5% sodium hypochlorite) |
Conejo |
N/A |
| Triton 100X |
Sigma-Aldrich |
Cat#T8787 |
| MURASHIGE & SKOOG MEDIUM + VITAMINS/MES |
Duchefa Biochemie |
Cat#M0255.0001 |
| Sucrose |
Sigma-Aldrich |
Cat#S7903 |
| PLANT AGAR |
Duchefa Biochemie |
Cat#P1001 |
| Potassium hydroxide |
Sigma-Aldrich |
Cat#P1767 |
| Recombinant protein bZIP67 |
This manuscript |
N/A |
| Trizma® base |
Sigma-Aldrich |
Cat#T1503 |
| Sodium chloride |
Sigma-Aldrich |
Cat#S7653 |
| EGTA |
Sigma-Aldrich |
Cat#E3889 |
| Immobilon-P Membran, PVDF, 0.45 μm |
Millipore |
Cat#IPVH00010 |
| cOmplete Protease Inhibitor Cocktail |
Roche |
Cat#04693124001 |
| Bio-Rad Protein Assay |
Bio-Rad |
Cat#5000001 |
| Tween® 20 |
Sigma-Aldrich |
Cat#P2287 |
|
| Critical commercial assays |
|
| RNeasy Plant Mini Kit |
QIAGEN |
Cat#74904 |
| ECL Advance Western Blotting Detection Kit |
GE Healthcare Life Sciences |
Cat#RPN2135 |
| QuikChange II Site-Directed Mutagenesis Kit |
Agilent |
Cat#200521 |
| Geneclean® Kit |
MP Biomedicals |
Cat# SKU: 111001200 |
|
| Deposited data |
|
| bZIP67 mass spectrometry proteomics |
This manuscript |
PRIDE: PXD048872
|
|
| Experimental models: Organisms |
|
| Col-0 |
NASC |
N1093 |
| bzip67 |
GABI-Kat |
GABI314DO4 |
| abi5-1 |
NASC |
N8105 |
| Nicotiana benthamiana |
Saavedra et al.55
|
N/A |
|
| Oligonucleotides |
|
| Primers |
This manuscript |
See Table S1
|
|
| Recombinant DNA |
|
| pENTR/D-TOPO |
Invitrogen |
Cat#K240020 |
| pEarleyGate 201 |
Earley et al.56
|
N/A |
| pETM11 |
EMBL Protein Expression and Purification Facility |
N/A |
| pGWB5 |
Nakagawa et al.57
|
N/A |
|
| Software and algorithms |
|
| ImageJ |
Schneider et al.58
|
https://imagej.nih.gov/ij/ |
| Office Excel Power Point 2016 |
Microsoft |
N/A |
| GPS-SNO 1.0 |
Xue et al.1 (SI REFERENCES) |
N/A |
| MaxQuant and Perseus software |
Wendrich et al.44
|
N/A |
| BioRender |
BioRender |
https://www.biorender.com/ |