FIG. 2.

Soft agar colony formation and morphology in adherent cultures of NIH 3T3 and Rat 6 cells that express H-Ras 12V and effector loop mutants. NIH 3T3 and Rat 6 cells that expressed neo or the indicated H-Ras constructs were cultured in soft agar or on tissue culture dishes (monolayer) as described in Materials and Methods and photographed. (A) Soft agar cultures. The photomicrographs demonstrate relative colony size; for colony-forming efficiencies, see Table 1. (B) Monolayer cultures of NIH 3T3 cells. Original magnification, ×40.