Figure 9.

Presynaptic measurements of calcium using jGCaMP8f fail to detect calcium transients at frequencies representing spontaneous release. A, Baseline, evoked, and DF of jGCaMP8f fluorescent signal are shown for three individual boutons, scale bar = 2 mm. B, Traces demonstrating the jGCaMP8f DF/F in response to nine single AP stimulations across three individual boutons located along the same axon. C, Example traces from bouton 1 (Fig. 9B) of jGCaMP8f in the absence of stimulation. D, Example traces from bouton 2 (Fig. 9B) of jGCaMP8f in the absence of stimulation. E, Example traces from bouton 3 (Fig. 9B) of jGCaMP8f in the absence of stimulation.