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. 2023 Sep 28;109(5):1348–1358. doi: 10.3324/haematol.2023.283178

Figure 2.

Figure 2.

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 protease activity in B-cell acute lymphoblastic leukemia cell lines and primary samples. Immunoblot analysis showing expression of mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) and its substrates (CYLD, HOIL, RelB) in (A) 5 pro B-cell acute lymphoblastic leukemia (B-ALL) cell lines; (B) 6 pre-B-ALL cell lines; (C) 6 mature B-ALL cell lines; (D) CD19-selected primary samples representing 6 B-ALL and one chronic lymphocytic leukemia sample. TMD8 and JURKAT were used as positive and negative controls, respectively, for substrate cleavage. (E) Immunoblot analysis showing inhibition of CYLD, RelB, and Regnase cleavage by MALT1 following a 12-h treatment with Z-VRPR-fmk in the mature B-ALL cell line 2F7. RAJI is shown as a negative control. (F) Cell-based reporter GloSensor assay showing intrinsic MALT1 proteolytic activity in the cell lines is shown. C-ter : cleaved substrates.