Table 2.

Summary of eIF4G-dependent translation assay activity

Figures	RNA template	λ-eIF4G Conc (μM)	λ-eIF4G682-1104 Luminescence (CPS)	λ-eIF4G682-1104 Relative translation	λ-eIF4G721-1104 Luminescence (CPS)	λ-eIF4G721-1104 Relative translation
Figure 2B	−5.4 kcal/mol	0	18 ± 2	0	17 ± 1	0
	boxB Reporter RNA	0.1	26 ± 2	0.5 ± 0.11	20 ± 1	0.23 ± 0.1
		0.2	94 ± 9	4.3 ± 0.54	33 ± 2	1.0 ± 0.14
		0.3	403 ± 14	21.7 ± 1.5	120 ± 14	6.2 ± 0.39
		0.4	649 ± 39	35.3 ± 1.6	214 ± 23	11.9 ± 0.93
		0.6	897 ± 91	48.9 ± 1.7	408 ± 55	23.3 ± 1.8
		0.8	1098 ± 97	60.1 ± 1.7	603 ± 58	35.2 ± 1.4
		1	963 ± 69	52.8 ± 2.6	624 ± 46	36.6 ± 1.5
		1.5	-	-	620 ± 70	36.1 ± 1.3
Figure 2C	−19.5 kcal/mol	0	18 ± 1	0	17 ± 1	0
	boxB Reporter RNA	0.1	24 ± 2	0.3 ± 0.01	22 ± 0.3	0.3 ± 2
		0.2	50 ± 14	1.8 ± 0.8	30 ± 2	0.8 ± 14
		0.3	172 ± 16	8.4 ± 0.9	91 ± 10	4.3 ± 16
		0.4	387 ± 18	20.2 ± 0.7	149 ± 9	7.7 ± 18
		0.6	600 ± 56	31.7 ± 0.5	296 ± 16	16.1 ± 56
		0.8	687 ± 72	40.3 ± 4.3	399 ± 8	22.1 ± 72
		1	829 ± 65	44.2 ± 0.3	376 ± 34	20.6 ± 65
		1.5	-	-	445 ± 84	24.8 ± 65

Translation activity in luminescence counts per second (CPS) is summarized for each boxB renilla luciferase reporter RNA tested with λ-eIF4G_682-1104 or λ-eIF4G_721-1104.