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. 2023 Nov 15;15(11):mjad068. doi: 10.1093/jmcb/mjad068

Figure 3.

Figure 3

The multiplexed TR-FRET assay allows the detection of SMAD4–SMAD3–DNA complex dynamics. (A and B) Bar graphs showing the PPI (A) and PDI (B) signals from cell lysates co-expressing His-SMAD4 and Flag-SMAD3 with 5′-FAM-SBE4 WT or Mut dsDNA. (C and D) Bar graphs showing the PPI (C) and PDI (D) signals from cell lysates co-expressing Flag-SMAD3 and His-SMAD4 WT or MH2 domain variants with 5′-FAM-SBE4 WT dsDNA. (E and F) Bar graphs showing the PPI (E) and PDI (F) signals from cell lysates expressing SMAD3 and SMAD4 WT or MH1 domain variants with 5′-FAM-SBE4 dsDNA. The data are expressed as mean ± SD from triplicates from three independent experiments. *P < 0.01, **P < 0.001.