TABLE 1.

DNA binding affinities of rSSRP1

Oligonucleotide	Kd (nM)e	DNA binding specificityf	Binding site sequenceg
Wild type	2.3	1.0	GATTCTGTTTTAACAGCTTT
−422 mutation	>240	<0.010	GATTCTGTTTTAACAGATTT
−425 mutation	>100	<0.023	GATTCTGTTTTAAAAGCTTT
Clustered mutationsa	>140	<0.016	CATTATCTTTAAAGAACTTT
−435 mutation	12.4	0.185	GATGCTGTTTTAACAGCTTT
−433 mutation	38	0.061	GATTCGGTTTTAACAGCTTT
V-(D)-J signalb	32	0.072	GATCCCACAGTGCTCCAGGGCTGAACAAAAACCGAATT
GATAc	>500	<0.005	GGGCAACTGATAAGGATTCC
MAD 7,8d	>500	<0.005	GAAACTAAGGTACAGAAGTT

^aThis oligonucleotide contains a cluster of six point mutations within PRE II (51). 

^bT160, the murine ortholog of SSRP1, was isolated from a cDNA expression library by using an oligonucleotide containing a murine V-(D)-J RSS (40). 

^cGATA binding site of the mouse α1-globin promoter (52). 

^dAn irrelevant DNA sequence upstream of the PRE II DNA recognition element of the ɛ-globin gene (50). 

^eIndividual point mutations were characterized previously for PREIIBF binding (15). A subset of these was used to calculate K_ds for rSSRP1. 

^fDefined as the ratio of the wild-type K_d to test sequence K_d. 

^gMutated nucleotides are underlined.