Fig. 1.

A) Schematic representation of four classical cell surface markers expressed in MSCs by enzymatic digestion method was validated by flowcytometry among freshly isolated and expanded MSCs. Illustrative histograms of surface marker expression compared to unstained MSCs (negative control). MSCs expressed CD44, CD73, CD90 and CD105, but did not express CD34 and CD45. B) MSCs have a spindle and fibroblast-like shape, illustrating the typical shape of MSCs and the U87 cell line has an epithelial morphology. Cells differentiated for 12 days have altered morphology and contains lipid drops. Differentiated cells stained with Oil Red O had well-defined lipid inclusions. Phase contrast microscopy allowed us to observe large lipid drops. Oil red O staining shows cells grown in the defined inductive medium differentiate into adipogenic lineages, for osteogenic differentiation induction was validated by alizarin red staining. Cells grown in a defined inductive medium differentiate into osteogenic lineages and finally various calcium depositions were seen. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)