Table 5. Percent Recoveries Associated with Cholesterol and 25-Hydroxycholesterol Measurements in Freshly Prepared Lab-Grade ALFQ.

accuracy studies for cholesterol measurements
	cholesterol conc. (μg/mL)
dilution of test samples	calculateda	theoretical	% recovery
1:150	10,252.55 ± 391.03	10,822.33	94.74 ± 3.61
1:300	12,185.10 ± 246.42	10,822.33	112.59 ± 2.28
1:600	11,419.40 ± 891.51	10,822.33	105.52 ± 8.24

accuracy studies for 25-hydroxycholesterol measurements
	25-hydroxycholesterol conc. (μg/mL)
spiked level	calculated	theoretical	% recovery
20%	2,197.35 ± 238.92	2,166.00	101.45 ± 11.03
10%	1,137.35 ± 114.23	1,083.00	105.02 ± 10.55
5%	435.45 ± 48.09	541.50	80.42 ± 8.88
0%	N.D.b

^aThe calculated concentrations are the actual measured concentrations after accounting sample dilutions.

^bN.D. = nondetected.