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DMS footprint analysis of Pho4 binding to UASp2. Binding of wild-type Pho4 and Pho4Δint to the upstream UASp2 element in the PHO5 promoter variant in which UASp1 was replaced by UASp2 (see schematic) was examined in YS22 (pho4) or YS27 (pho2 pho4). For details of DMS footprinting and the primer used (arrow in the schematic), see Materials and Methods.
