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Mutant Pho4 protein lacking the Pho2 interaction domain (Pho4Δint) can activate UASp2 but not UASp1
| Reporter | β-Galactosidase activity (U)a
|
|
|---|---|---|
| Pho4Δint | Wild-type Pho4 | |
| PHO5-lacZ | 55 | 920 |
| UASp1 CYC1-lacZ | <5 | 622 |
| UASp2 CYC1-lacZ | 99 | 199 |
The activity of the PHO5 promoter and of heterologous promoter constructs containing individual PHO5 UAS elements was measured in strains expressing either wild-type Pho4 or Pho4Δint. Activity values with the parent vector CYC1-lacZ were <5U.
