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(A,B) 3D confocal fluorescence microscopy reconstructions of (GFP-expressing) MCF7 cells cultured on scaffolds coated with fibronectin and collagen, after 4 (A) and 21 (B) days in vitro (DIV) (Scale bars: 500 μm). (C) SERS spectra of AuNR@2NAT in the scaffold and AuNS@4BPT in labeled MCF7 cells. (D) 3D SERS reconstruction of MCF7 cells labeled with AuNS@4BPT (blue) on a scaffold labeled with AuNR@2NAT (red) after 5 DIV. The cube dimensions are 2 × 2 × 1 mm3 (scale bars: 500 μm).
