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. 2024 Apr 18;18(17):11257–11269. doi: 10.1021/acsnano.4c00543

Figure 3.

Figure 3

Monitoring of spheroidal growth (MCF7 in red, HDF in green) in a PEGDA scaffold over time. (A) Schematic view of the preparation of the scaffold containing AuNR@2NAT, optimized ECM, and SERS-labeled MCF7 and HDF cells and spheroids. (B) MIP from Z-stack confocal fluorescence imaging of spheroid growth after 1 DIV. Scale bar: 1000 μm. (C) Confocal microscopy images at different times of the spheroid found in the yellow square in (B). Scale bars: 50 μm. (D) Merged XY SERS maps showing spheroid growth within a scaffold labeled with AuNR@2NAT (cyan), MCF7 cells labeled with AuNS@BT (red), and HDF cells labeled with AuNS@4BPT (green). Scale bars: 500 μm.