Fig. 5.

PCED1B-AS1 mediated MAP2K7 to affect the function of gastric cancer cells. A RT-qPCR analysis was performed to detect the mRNA expressions of MAP2K7 in gastric cancer cells. B RT-qPCR analysis was performed to detect the mRNA expressions of MAP2K7 in different groups. C Western blotting assay was used to detect the protein expressions of MAP2K7 in different groups. D After transfection with si-PCED1B-AS1 and oe-MAP2K7, the mRNA expression of MAP2K7 was detected by RT-qPCR analysis. E The protein expressions of MAP2K7 in different groups were detected by western blotting assay. F Cell viability was assessed by CCK-8 kit. G Transwell experiment was used to detect the invasion in different groups. H Wound healing assay was used to detect the migration in different groups. The expressions of E-cadherin, N-cadherin and Vimentin in different groups were detected by western blotting assay. The data were presented as mean ± SEM, versus si-NC group, *P < 0.05, **P < 0.01; versus si-PCED1B-AS1 group, ^#P < 0.05, ^##P < 0.01, n = 3