Fig. 8.

PCED1B-AS1 mediated miR-3681-3p/MAP2K7 signaling axis to regulate gastric cancer cell function. A RT-qPCR analysis was used to detect the mRNA expressions of MAP2K7 in AGS and HGC-27 cells. B Western blotting assay was performed to detect the expressions of MAP2K7 in AGS and HGC-27 cells. C The cell viability was assessed by CCK-8 kit. D Transwell experiment was performed to detect the invasion in AGS and HGC-27 cells. E Wound healing assay was used to detect the migration in AGS and HGC-27 cells. F The expressions of E-cadherin, N-cadherin and Vimentin in AGS and HGC-27 cells were detected by western blotting assay. The data were presented as mean ± SEM, versus mimics NC group, *P < 0.05, **P < 0.01; versus si-NC + miR-3681-3p inhibitor group, ^#P < 0.05, ^##P < 0.01; versus si-MAP2K7 + inhibitor NC group, ^&P < 0.05, n = 3