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. 1998 May;18(5):2650–2658. doi: 10.1128/mcb.18.5.2650

FIG. 2.

FIG. 2

FIG. 2

FIG. 2

Characterization of the minimal activation domain for Pol II and Pol III snRNA promoters. (A) Schematic structures of the various truncated and fused Staf-Krox-20 proteins. The Krox-20 DBD is hatched. (B) Xenopus microinjection assay of transcriptional activation from the 3E.tRNASec promoter by the effectors depicted in panel A. Identities of the effector proteins are indicated above each lane. Lanes 9, 10, and 19, assays without effector (−). Positions of the 5S maxigene and tRNASec RNAs are indicated. Autoradiographs corresponding to lanes 1 to 9, 10 to 15, 16 to 18, and 19 to 20 were from separate experiments. (C) Xenopus microinjection assay of transcriptional activation from the 3E.U1 promoter by the effectors depicted in panel A. Positions of the U1 and 5S maxi RNAs are indicated. Lane 1, assay without effector (−).