Fig. 2.

Sensitivity of conditional reprogrammed human limbal epithelial cells and human airway epithelial cells to SARS-CoV-2 infection. A and B. Conditional reprogrammed human limbal epithelial cells and human airway epithelial cells were infected with WIV-04 strain of SARS-CoV-2 for 4 days. The transcript of SARS-CoV-2 spike gene in supernatant and the expression of SARS-CoV-2 nucleocapsid (NP) in cells were measured by RT-qPCR (A) and Immunofluorescence assay (B) respectively. Scale bar: 200 μm. C. Syncytia formation by fusion of SARS-CoV-2 spike expressing HEK293 cells and ACE2-expressing cells. HNCEC/HL-008, HAWEC-02014 and Vero cells were co-cultured with SARS-CoV-2 Spike-GFP expressing HEK293 or GFP expressing HEK293 cells at rate 2:1 for 96 h, and then fixed with 4 % PFA and strained with DAPI for microscope observation Scale bar: 200 μm.