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The increase in c-fos mRNA levels during contraction requires extracellular Ca2+. Stressed collagen matrices were preincubated for 10 min with 3 mM EGTA and/or 3 mM CaCl2 as shown. Subsequently, the matrices were released to initiate contraction. After 50 min, RNAs were isolated from the matrices and analyzed by Northern hybridization with c-fos and GAPDH probes.
