FIG. 3.

SKI6 does not affect stability of electroporated luciferase mRNA. Labeled mRNA was electroporated into wild-type and ski6-2 cells. The kinetics of luciferase synthesis (Fig. 4) and mRNA degradation were determined on portions of the same samples taken at 0, 10, 20, and 30 min. Extracted RNA was analyzed by agarose gel electrophoresis and autoradiography as described previously (31).