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ski6-2 cells show a novel species of rRNA whose origin is 25S rRNA. Polysome profiles of ski6-2 (ski6−) and wild-type (SKI6+) cells grown at either 30 or 39°C are shown. The A260 is plotted on the vertical axis. Northern analysis of fractions from polysome profiles at 30°C were made by using probes for 25S (p25S), 18S (p18S), or 5.8S (p5.8S) rRNAs (see Materials and Methods).
