FIG. 7.

(A) Long-term centrifugation on sucrose gradients allows a better separation of the novel 38S species from the usual 40S peak. The A₂₆₀ is plotted on the vertical axis. Northern analysis of fractions from polysome profiles at 39°C was done by using probes against the 25S (p25S) and 18S (p18S) rRNAs (see Materials and Methods). The dashed lines show the points on the UV scan corresponding to the fractions analyzed by Northern hybridization. (B) Hybridization of the blots from panel A with probes specific for either the 5′ end (p25S5′) or 3′ end (p25S3′) of 25S rRNA.