TABLE 1.
Translation of electroporated luciferase mRNAsa
| Strain | Expt no. | Luciferase activityb for phenotype of:
|
|||
|---|---|---|---|---|---|
| C+ A+ | C+ A− | C− A+ | C− A− | ||
| ski6-2 pSK16 (SKI+) | 1 | 39.5 | 1.22 | 9.05 | 0.46 |
| 39.6 | 1.07 | 9.22 | 0.38 | ||
| 39.8 | 1.14 | 9.15 | 0.37 | ||
| 2 | 39.6 | 1.35 | 11.0 | 0.43 | |
| 39.8 | 1.32 | 11.4 | 0.45 | ||
| 39.8 | 1.31 | 11.4 | 0.45 | ||
| 3 | 41.4 | 1.17 | 8.9 | 0.34 | |
| 42.5 | 1.23 | 9.2 | 0.32 | ||
| Avg | 40.4 | 1.22 | 9.8 | 0.39 | |
| ski6-2 pRS316 (ski6) | 1 | 32.9 | 9.3 | 11.1 | 2.31 |
| 33.1 | 9.3 | 11.1 | 2.29 | ||
| 33.1 | 9.4 | 11.1 | 2.20 | ||
| 2 | 29.4 | 10.5 | 14.3 | 2.26 | |
| 27.5 | 10.5 | 14.0 | 2.35 | ||
| 27.5 | 10.5 | 14.0 | 2.35 | ||
| 3 | 30.4 | 8.6 | 9.6 | 2.28 | |
| 31.2 | 9.0 | 10.1 | 2.31 | ||
| Avg | 30.6 | 9.6 | 11.7 | 2.29 | |
| ski6/SKI+ ratioc | 0.76 (1.0) | 7.9 (10.4) | 1.19 (1.56) | 5.9 (7.8) | |
a
Strain RV493 (K2−) carrying either pSKI6 (SKI+) or the vector pRS316 (ski6) was grown at 30°C in H-Ura and electroporated with 2 μg of luciferase mRNAs prepared with (C+) and without (C−) 5′ cap and with (A+) or without (A−) 3′ poly(A) as described in Materials and Methods.
b
Luciferase activity is expressed in light units per microgram of protein. The blank is <0.01 light unit.
c
This row of data shows the ski6/SKI+ ratio normalized on the assumption that this ratio is 1.0 for C+ A+ mRNA.