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. 2024 May 2;25(5):e13463. doi: 10.1111/mpp.13463

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© 2024 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Overexpression of AVR_1A and BEC1016 or RNAi of the target HvERdjB3 cause endoplasmic reticulum (ER) retention of a vacuolar marker. The vacuolar marker construct p35S::SP‐RFP‐AFVY was co‐expressed with either the pUbi::GW empty vector (EV), pUbi::AVR_A1 or pUbi::BEC1016 (both without signal peptide), along with either the control RNAi construct (hrpE‐RNAi) or an RNAi construct for HvERdj3B, in barley epidermal cells upon particle bombardment. The vacuolar marker (SP)‐RFP‐AFVY was partially mislocalized in an ER‐like structure after overexpression of AVR_A1 and BEC1016 or after RNA‐interference of HvERdj3B. This was evident by its presence in a perinuclear ring and occasionally in reticular structures. Counts indicate occurrence of mislocalization of the vacuolar marker in 30 cells. Scale bars, 10 μm.