FIG. 1.

Schematic representation of rad12⁺. (a) Alignment of Rad12p and BLMp sequences, showing the conserved acidic (A), basic (B), RecQp-like helicase (H), and extended high-level-homology domains. The acidic and basic regions exhibit greater than 30% acidic or basic residues over a span of at least 20 amino acids. The highly conserved helicase domain has been previously defined (13). The extended region of high-level homology is a region of 37% identity (57% similarity) that extends for an additional 199 amino acid residues past the C terminus of the helicase domain. (b) Corresponding schematic representation of the rad12⁺ gene and the construct used to generate the gene disruption, rad12::ura4⁺ (Sp359).