TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide name | Sequence | Locationa |
|---|---|---|
| BLS1 | CGA AAA TGT CAC TAG CCC CA | 68–87 |
| BLS2 | TTC GCT GAC GAT TGG GTT AG | 587–568 |
| BLS3 | TGG CCA TAG ATG ACA GCA GA | 3397–3416 |
| BLS4 | TTC CGT TGA CCA TCC ACT TC | 3757–3738 |
| BLS5 | GAC ATC TGC AGC GGC TGT TGG AAT T | (−20)–(−6)b |
| BLS6 | ACT CAT CAT TAA TCG GAT CC | 1290–1271 |
| UVDE1 | ATG CTT AGG CTA TTG A | 121–136 |
| UVDE2 | CAA CAG ACT CAT CAA T | 508–493 |
| UVDE7 | TCA AAA AGT ATG ACG | 1940–1926 |
| UVDE10 | CAA GCT GGC AAA TAA | 1143–1157 |
| LEU1 | GAG GAA TAT CCT CAC C | 631–647 |
| LEU2 | TAT CAG CGG TAG AAG C | 1074–1058 |
a
Locations of the primers are given with respect to the initiating ATG of the respective genes. Numbering in the location column is from the 11th nucleotide to the end.
b
The 10 nucleotides at the 5′ end of this oligonucleotide do not correspond to the rad12+ sequence but rather generate a Pst1 restriction site.