Table 4. Steady-state anisotropy values of free dyes and dyes which were attached to different proteins.

	Condition*	Alexa Fluor 555†	Alexa Fluor 647†
Free dye	50 mM KCl	0.21	0.16
Free dye	300 mM KCl	0.21	0.16
MalE-T36C-S352C	300 mM KCl	0.26	0.22
OpuA-K521C	50 mM KCl	0.30	0.28
OpuA-K521C	300 mM KCl	0.29	0.27
OpuA-N414C	50 mM KCl	0.28	0.24
OpuA-N414C	300 mM KCl	0.28	0.23

^*All conditions contained 50 mM HEPES-K pH 7.0.

^†Standard deviation between triplicates was less than 0.001.