Table 5. Primers used in this study to generate mutations in OpuA.

Oligo nr.	Oligo name	Sequence (from 5' to 3') *
9053	UOpuAC-K521C fw	aaaggttUgaagTGTgaaaatccagaagcttataaag
9054	UOpuAC-K521C rev	aaaccttUacgaacaatgg
9511	UOpuAC-N414C fw	aattgaagaUttaacaaatcaagc
9512	UOpuAC-N414C rev	atcttcaatUgaattaacACAcatataacttggaac
9617	UOpuAA-E190Q fw	aagctttcUctgctcttgac
9618	UOpuAA-E190Q rev	agaaagcTUGatccatgagcaaaatc
9619	UOpuAC-V149Q fw	atttgatUccagcgCAAgcattctttgg
9620	UOpuAC-V149Q rev	aatcaaaUaaacgaaaccagg

^*Mutated triplets and introduced uracils are in capitals.