Supporting Figure S2.

ORMDL1 and ORMDL2 expression does not affect IL-1β level in human MDMs.A, human MDM cells were transfected with full length Myc tagged ORMDL3 for 24 h and assessed for overexpression efficiency of myc-ORMDL3 in human monocyte derived macrophages by western blot. GAPDH was used as loading control. B, human MDMs were transfected with scrambled or siORMDL3 for 24 h and assessed for relative mRNA fold change of ORMDL3 showing knockdown efficiency by qRT PCR (n = 6 donor). C, human MDM were transfected with scrambled or siORMDL1 for 24 h, then stimulated with LPS ( 200 ng/ml) and Nigericin for 6 h and analysed for IL-1β secretion in the cell supernatant of (n = 5 donor). D, human MDMs were transfected with scrambled or siORMDL1 for 24 h and assessed for relative mRNA fold change of ORMDL1 showing knockdown efficiency by qRT PCR (n = 6 donor). E, human MDM were transfected with scrambled or siORMDL2 for 24 h ,then stimulated with LPS (200 ng/ml) and Nigericin for 6 h and analysed for IL-1β secretion in the cell supernatant of n = 5 donor. F, human MDMs were transfected with scrambled or siORMDL2 for 24 h and assessed for relative mRNA fold change of ORMDL2 showing knockdown efficiency by qRT PCR (n = 6 donor). Mean ± SEM, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 as determined by 1-way ANOVA for Figure 2, C and E and 2-tailed t test for rest of the figure. “ns” denotes non-significant.