Table 1.

Quantification of BL1.2 in stomach content extracted from piglets

		BL1.2 quantity detected in stomach extract using Octet
		1	2	3	Mean	Standard error of mean (SEM)
nM BL1.2	Piglet (no product)	ND	ND	13.7	–	–
	Piglet 1 (BL1.2)	376	383	284	347.67	31.90
	Piglet 2 (BL1.2)	227	485	525	412.33	93.38
µg/mL BL1.2	Piglet (no product)	ND	ND	0.0382	–	–
	Piglet 1 (BL1.2)	10.487	10.682	7.921	9.70	0.89
	Piglet 2 (BL1.2)	6.331	13.527	14.643	11.50	2.61

Piglets were fed a standard Danish sow diet and received BL1.2 via gauvage. Binding affinity of BL1.2 in stomach extracts to chip-attached FaeG (F4⁺ ETEC tip adhesin) as determined using Octet measurements. Samples were measured in triplicate, and signals measured in nM was translated to µg/mL based on the molecular weight of BL1.2.