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. 2024 May 2;10:214. doi: 10.1038/s41420-024-01982-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B The effect of NETs on the expression of IL-6, NLRP1, NLRP2, NLRP3, NOD2 and PLCG1 in Cal27 and HN6 cells was detected by RT-qPCR. C The effect of NETs on the expression of NLRP3 in Cal27 and HN6 cells was detected by western blot. D The effect of NETs on the gasdermin cleavage level in Cal27 and HN6 cells was detected by western blot. E, F Representative bright-field microscopy images of Cal27 and HN6 cells after the treatment of NETs; the arrows indicated pyroptotic cells. (Scale bars = 200 μm). G, H Pyroptosis index of Cal27 and HN6 cells after treatment (n = 4). (ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001). NET neutrophil extracellular trap, OSCC oral squamous cell carcinoma.