FIG. 4.

Localization of the E(z) domain that mediates binding to esc protein. GST, GST-E(z) (full length, amino acids 1 to 760), and GST-E(z) deletion derivatives were tested for binding to radiolabeled esc protein produced by in vitro translation (A) or HA-esc protein from embryo extracts (B). The GST fusion proteins used are indicated above the lanes; the numbers refer to E(z) amino acids included in each fusion protein. (A) Autoradiograph of SDS gel. (B) Western blot probed with anti-HA antibodies. Lanes labeled “Input” and “Sup.” are as described in the legend to Fig. 3. Numbers at left of panels are kilodaltons.