Fig. 4.

The overall changes in gut microbiota (GM) in rats were observed. Class 1: Control group; Class 2: ICCP group; Class 3: ICCP + ZBDH group; Class 4: ICCP + GnRHa group; (A) The dilution curve was used to analyze the abundance of species in different samples; (B) the Rank-Abundance curve was used to analyze the sample diversity; (C) alpha diversity analysis was performed; beta diversity analysis, including (D) analysis of similarity (ANOSIM), (E) principal coordinate analysis (PCA), and (F) non-metric multidimensional scaling (NMDS) were performed to analyze the differences of microbial community among groups.