Fig. 3.

Respiration and ROS production in isolated kidney and brain mitochondria. Mitochondria isolated from kidney (A, B) or brain (C, D) were incubated as in Fig. 1A. Panels A and C show exemplar H₂O₂ traces ± FCCP or ± rotenone. (E) The rate of H₂O₂ production after addition of succinate to the closed chamber is shown for heart, kidney and brain mitochondria. (F) The rate of H₂O₂ production over the first 10 min following reoxygenation is shown for heart, kidney and brain mitochondria. * = p < 0.05, **** = p < 0.0001. Data are means ± SEM of n = 9–28 experiments each on a separate mitochondrial preparation.