Fig. 5.

Effects of mitochondria-acting compounds on RET ROS in vitro. (A–D) Mitochondria isolated from the heart were incubated under the same conditions as described in Fig. 1A in a 96 well plate. Concentrations of malonate and metformin (0–10 mM, A), butylmalonate (0–20 mM, B), MitoQ and S1QEL 1.1 (0–5 μM, C) and Cyclosporin A (0–1.6, D) were assessed. The mean slopes ± SD for the rate of Amplex Red oxidation were determined in quadruplicate technical replicates at each concentration. (E) From the experiments shown in A-D the effects of malonate (10 mM), butylmalonate (20 mM), metformin (10 mM), S1QEL 1.1 (2.5 μM), MitoQ (2.5 μM) and Cyclosporin A (1.6 μM) were assessed on the rate of H₂O₂ production over the first 10 min following reoxygenation compared with control conditions. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001. Data for (E) are means ± SEM of n = 3–12 experiments each on a separate mitochondrial preparation.