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Kinetics for the phosphorylation of Axil(265-483) by GSK-3β. (A) Time course. MBP-Axil(265-483) (200 ng of protein) purified from E. coli was incubated with (•) or without (○) GST–GSK-3β (100 ng of protein) for the indicated periods of time. (B) Dose dependency. The indicated concentrations of MBP-Axil(265-483) were incubated with GST–GSK-3β (100 ng of protein) for 20 min. The results shown are representative of three independent experiments.
