Fig. 3.

Functional enrichment analysis and RT-qPCR validation. (A) Dotplot for the results of GO-based functional enrichment analysis. “GeneRatio” equals the number of DEGs divided by the total number of genes in the pathway. The dot size stands for the number of DEGs in the pathway and the dot color stands for the p‐value. (B) Dotplot for the results of KEGG-based functional enrichment analysis. The definitions of “GeneRatio”, dot size and color are the same as in (A). (C) RT-qPCR validation for 10 DEGs identified by translatome sequencing. The left and right panels show the RT-qPCR results of genes up-regulated and down-regulated in translatome after sevoflurane exposure, respectively. Fold change has been normalized with respect to the control. “NS” indicates no statistical difference, and those without “NS” label have statistical difference. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)