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. 2024 Apr 12;10(9):e28876. doi: 10.1016/j.heliyon.2024.e28876

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© 2024 Published by Elsevier Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Differential expression analysis and RT-qPCR validation. (A) Volcano plot of the transcriptome-based DEGs between Group A and B, Group B and the control. Red points stand for up-regulated DEGs and blue ones stand for down-regulated DEGs. (B) RT-qPCR validation for 10 DEGs identified by transcriptome sequencing. The left and right panels show the RT-qPCR results of genes up-regulated and down-regulated in transcriptome after sevoflurane exposure, respectively. Fold change has been normalized with respect to the control. “NS” indicates no statistical difference. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)