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. 2024 Apr 23;10(9):e29829. doi: 10.1016/j.heliyon.2024.e29829

Fig. 3.

Fig. 3

Inhibition of ST3GAL4 hinderd tumor cell growth and cell cycle progression. A: Protein immunoblotting was used to detect the expression levels of ST3GAL4 in U87 and U251 glioma cells. B: Stable U251 cell lines expressing control or ST3GAL4-targeting shRNAs. C: The efficiency of ST3GAL4 knockdown at the mRNA level was assessed using qPCR. D: Protein immunoblotting was used to determine the efficiency of ST3GAL4 knockdown. E, F: The proliferation of stable U251 cells was measured using CCK-8 assay (E) and clonal formation assay (F). G: Apoptosis assay by flow cytometry with U251 cells knocked down for ST3GAL4. H: Cell cycle assay by flow cytometry with U251 cells knocked down for ST3GAL4. *P < 0.05, **P < 0.01, ***P < 0.001.