Fig. 7.

Correlation between ST3GAL4 and immune checkpoint genes as well as macrophage immunosuppressive markers. A: Heatmap displaying the expression of immune checkpoint genes in groups with high and low ST3GAL4 expression. B–E: Correlation analysis of ST3GAL4 with immune checkpoint gene PDCD1(B), CD274 (C), PDCD1LG2 (D), and HAVCR2 (E). F, G: Expression of CD163 (F) and CD274 (G) on macrophages cocultured with or without U251 cells. Flow cytometry was used to detect CD163 and CD274 in unstained control samples as well as in macrophages alone, macrophages cocultured with control U251 cells, or macrophages cocultured with sh-ST3GAL4 U251 cells for 24 h.