TABLE 1.

S. cerevisiae strains^a

Strain	Genotype	Reference or source
L5783	MATa ura3-52	18
	MATα ura3-52
HLY351	MATa ura3-52 ste7Δ::LEU2	18
	MATα ura3-52 ste7Δ::LEU2
HLY352	MATa ura3-52 ste12Δ::LEU2	18
	MATα ura3-52 ste12Δ::LEU2
HLY492	MATa ura3-52 ste20Δ::LEU2	18
	MATα ura3-52 ste20Δ::LEU2
HLY506	MATa ura3-52 ste11Δ::LEU2	18
	MATα ura3-52 ste11Δ::LEU2
SC110	MATa ura3-52 ash1-Δ1::hisG-URA3-hisG	This work
	MATα ura3-52 ASH1
SC112	MATa ura3-52 ash1-Δ1::hisG-URA3-hisG	This work
SC113	MATα ura3-52 ash1-Δ1::hisG-URA3-hisG	This work
SC114	MATa ura3-52 ASH1	This work
SC121	MATa ura3-52 ash1-Δ1::hisG	This work
SC122	MATα ura3-52 ash1-Δ1::hisG	This work
SC125	MATa ura3-52 ash1-Δ1::hisG	This work
	MATα ura3-52 ash1-Δ1::hisG
SC126	MATa ura3-52 GFP-ASH1::URA3::ash1-Δ1::hisG	This work
	MATα ura3-52 ash1-Δ1::hisG
SC127	MATa ura3-52 ash1-Δ1::hisG leu2-Δ1	This work
SC128	MATα ura3-52 ash1-Δ1::hisG leu2-Δ1	This work
SC135	MATa ura3-52 ash1-Δ1::hisG leu2-Δ1 ste12Δ::LEU2	This work
SC136	MATα ura3-52 ash1-Δ1::hisG leu2-Δ1 ste12Δ::LEU2	This work
SC137	MATa ura3-52 ash1-Δ1::hisG leu2-Δ1 ste12Δ::LEU2	This work
	MATα ura3-52 ash1-Δ1::hisG leu2-Δ1 ste12Δ::LEU2

^aAll strains are isogenic with strain Σ1278b, which is ho⁻ and unable to switch mating types.