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3BA and 3BD mRNAs are unstable, while 3BB and 3BC mRNAs are stable. Polyadenylated RNAs prepared from 0- to 1-, 1- to 2-, 2- to 3-, and 3- to 4-h-old embryos from 3BA, 3BB, 3BC, and 3BD transgenic flies were analyzed on Northern blots hybridized with bicoid and rpA1 mRNA probes. rpA1 mRNA served as a loading control. mRNA sizes: bicoid mRNA, 2.6 kb; 3BA mRNA, 1.2 kb; 3BB mRNA, 1.8 kb; 3BC mRNA, 1.4 kb; 3BD mRNA, 1.6 kb; and rpA1 mRNA, 0.6 kb.
