TABLE 1.

Oligonucleotides used in 3B deletion derivative construction, RNase H treatment, and BIE constructs^a

Oligonucleotide	Sequencea	Restriction enzyme	Gene (positions)
3BA upper strand	5′ GGCGCTAGCAATTAGCCTGGACGAGAGGCGT 3′	NheI	bicoid (4007–4029)
3BA or 3BD lower strand	5′ GATTACGCCCAAGAGAAACATT 3′		bicoid (4473–4452)
3BB or 3BC upper strand	5′ CGACTACGCCGCTAGCAGTTCG 3′		HA1 DNA cassette (47–65), bicoid (2765–2767)
3BB lower strand	5′ GGGACGCGTCTAATTGAAGCAGTAGGCAAAC 3′	MluI	bicoid (4012–3991)
3BC lower strand	5′ GGGACGCGTCTATCCGCCGATGCCGATGCCACAC 3′	MluI	Stop codon; bicoid (3136–3115)
3BD upper strand	5′ GCCGCTAGCCCTTGCGCCATCGCCGTTGGCG 3′	NheI	bicoid (3137–3158)
ΔNRE	5′ GTACAATCAGGAACAACAGTGGT 3′		bicoid (4079–4057)
BIES	5′ CCGGCCCTGGACGAGAGGCGTGTTAGAGAGTTTCATTAGCTTTAGGTTG 3′	NgoMI	bicoid (4013–4055)
BIEA	5′ CCGGCAACCTAAAGCTAATGAAACTCTCTAACACGCCTCTCGTCCAGGG 3′	NgoMI	bicoid (4055–4013)

^aUnderlining indicates added nucleotides. Boldface type indicates endonuclease restriction sites.