KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human IgG (Fab-specific) horseradish peroxidase antibody	Sigma-Aldrich	Cat# A0293; RRID: AB_257875
Primary Antibody – Anti RBD mAb generated in house (CR3022)	Tian et al.31	N/A
Biological samples
Sera from PARIS study participants (adults)	This Paper, Icahn School of Medicine Personalized Virology Initiative PARIS Study	IRB-20–03374; STUDY-20–00442
Chemicals, peptides, and recombinant proteins
Recombinant SARS-CoV-2 spike receptor binding domain (RBD)	Krammer laboratory at the Icahn School of Medicine at Mount Sinai This protein has been used in other studies and was produced as previously described at Amanat et al.26	https://labs.icahn.mssm.edu/krammerlab/reagents/
Recombinant SARS-CoV-2 spike (S)	Krammer laboratory at the Icahn School of Medicine at Mount Sinai This protein has been used in other studies and was produced as previously described at Amanat et al.26	https://labs.icahn.mssm.edu/krammerlab/reagents/
SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride)	Sigma-Aldrich	Cat# P9187
3-molar hydrochloric acid	Thermo Fisher Scientific	Cat# S25856
Non-Fat Dry Milk Omniblok	American Bio	Cat# AB10109–01000
Deposited data
Figure Source Data (F1-F4) Antibody titers (AUC) measured by ancestral full-length spike-binding ELISA Participant demographic information	This Paper	ImmPort SDY2468
Software and algorithms
GraphPad Prism v10	GraphPad Software Inc.	RRID:SCR_002798
Python Programming Language v3.9.17	Python Software Foundation	RRID:SCR_008394
MatPlotLib v3.7.1	Hunter32	RRID:SCR_008624
seaborn v0.12.2	Waskom33	RRID:SCR_018132
NumPy v1.24.3	Harris et al.34	RRID:SCR_008633
SciPy v1.11.1	Virtanen et al.35	RRID:SCR_008058
Statistical Analysis System v9.4 (SAS)	SAS Institute Inc.	RRID:SCR_008567
Non-linear mixed effects model (SAS code)	This Paper	https://doi.org/10.5281/zenodo.10467603
REDCap 13.1.37 - © 2024	Vanderbilt University	RRID:SCR_003445
Canva® Canva Pro		https://www.canva.com/