Table 2. Comparison of the Experimentally Measured Electrophoretic Mobilities from Previous Studies with Our Model Predictions.
| speciesa | buffer type | pH | σb (mC/m2) | λ (nm) | rA (nm) | rB (nm) | L (nm) | qA | qB | paper mobilityc,d | predicted mobilityd | refs. |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TR-DHPE (ortho) | 1 mM NaH2PO4 and 5 mM NaCl | 4.9 | –2.54 | 3.4 | 0.5 | 0.4 | 2 | –1 | 0 | –7.6 | –4.3 | (12) |
| TR-DHPE (ortho) | 0.5 mM NaH2PO4 buffer w/o NaCl | 4.9 | –2.54 | 10 | 0.5 | 0.4 | 2 | –1 | 0 | –6.0 | –3.0 | |
| TR-DHPE (para) | 1 mM NaH2PO4 and 5 mM NaCl | 4.9 | –2.54 | 3.4 | 0.3 | 0.4 | 2 | –1 | 0 | –7.0 | –6.3 | |
| TR-DHPE (para) | 0.5 mM NaH2PO4 buffer w/o NaCl | 4.9 | –2.54 | 10 | 0.3 | 0.4 | 2 | –1 | 0 | –7.0 | –5.6 | |
| pR + Alexa488 | DI water | N.A. | –1.00 | 1000 | 0.5 | 5.7 | 5 | –2.00 | –4.76 | –0.3 | –0.2 | (24) |
| CymA + ATTO565 | DI water | N.A. | –1.00 | 1000 | 2 | 1.8 | 5 | 1.11 | –0.48 | 2.2 | 7.8 | (25) |
| StrA + Alexa488*0.3 | 0.5 mM sodium citrate/0.5 mM tris buffer + 0 mM NaCl | 7.9 | –3.50 | 10 | 2 | 0.4 | 2 | –3.07 | –2 | –1.9 | –1.9 | |
| StrA + Alexa488*0.3 | 0.5 mM sodium citrate/0.5 mM tris buffer + 5 mM NaCl | 7.9 | –3.50 | 4 | 2 | 0.4 | 2 | –3.07 | –2 | –5.0 | –6.2 | |
| StrA + Alexa488*0.3 | 0.5 mM sodium citrate/0.5 mM tris buffer + 10 mM NaCl | 7.9 | –3.50 | 3 | 2 | 0.4 | 2 | –3.07 | –2 | –5.8 | –7.6 | |
| StrA + Alexa488*4 | 1 mM sodium citrate buffer | 4.2 | –2.26 | 11.4 | 2.4 | 0.4 | 2 | –3.31 | 0 | 6.7 | 3.4 | (16) |
| StrA + Alexa488*4 | 1 mM tris buffer | 7.5 | –3.36 | 10.1 | 2.4 | 0.4 | 2 | –6.14 | –2 | –6.0 | –9.2 | |
| StrA + Alexa488*4 | 1 mM tris buffer | 9.4 | –4.10 | 12.0 | 2.4 | 0.4 | 2 | –7.76 | –2 | –8.8 | –9.6 | |
| StrA + Alexa488*0.3 | 1 mM sodium citrate buffer | 4.2 | –2.26 | 11.4 | 2 | 0.4 | 2 | 0.09 | 0 | 9.0 | 13.2 | |
| StrA + Alexa488*0.3 | 1 mM tris buffer | 7.5 | –3.36 | 10.1 | 2 | 0.4 | 2 | –2.74 | –2 | –1.4 | –1.4 | |
| StrA + Alexa488*0.3 | 1 mM tris buffer | 9.4 | –4.10 | 12.0 | 2 | 0.4 | 2 | –4.36 | –2 | –2.5 | –2.9 |
a
TR: Texas Red; pR: proteorhodopsin; StrA: streptavidin.
b
Ref (40).
c
Electrophoretic mobility from previous studies.
d
(×10–3 (μm/s)/(V/cm)).