Figure 2.

Macro-regions, micro-regions and cell-to-cell distance. (A) The background defined based on a user-specified distance from the tumor boundary is removed from the whole-slide image. The remaining ROI is partitioned into tumor region, tumor border, stroma border and stroma region (macro-regions). (B) Within the tumor region, there are small regions of interest, defined as micro-regions, which allow fine-grained analyses. (C) The distance of a cell (e.g. a tumor cell) to another cell type (e.g. immune cells) is defined as the distance between the cell and its nearest neighbor of another cell type.