| Antibodies |
|
| anti-CD320 |
Proteintech |
RRID:AB_2074223 |
| anti-Lcn2 |
R&D Systems |
RRID:AB_355022 |
| anti-Hmox1 |
ThermoFisher Scientific |
RRID:AB_2544814 |
| anti-GFAP |
Neuromics |
RRID:AB_10014322 |
| Alexa Fluor 488 |
ThermoFisher Scientific |
RRID:AB_143165 |
| Alexa Fluor 568 |
ThermoFisher Scientific |
RRID:AB_2534098 |
| anti-NeuN |
MilliporeSigma |
RRID:AB_2571567 |
| anti-rabbit APC |
ThermoFisher Scientific |
RRID:AB_429727 |
| HRP conjugated anti-rabbit or anti-mouse IgG(H + L) |
Cell Signaling Technologies |
RRID:AB_2099233 |
|
| Biological samples |
|
| Human MS and control brain |
Rocky Mountain MS Center Tissue Bank |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| MOG35–55
|
EZBiolab |
cat # cp7203–5 |
| Pertussis toxin |
List Biological Laboratories |
cat # 180 |
| Vitamin B12
|
Sigma |
cat #V2876 |
| Recombinant human CD320 |
R&D Systems |
cat # 1557-CD-050 |
| Recombinant human TCN2 |
R&D Systems |
cat # 7895-TC-050 |
| FTY720 |
Novartis |
N/A |
| FTY720-P |
Novartis |
N/A |
| Sphingosine |
Avanti Polar Lipids |
cat # 860490P |
| S1P |
Avanti Polar Lipids |
cat # 860492P |
|
| Critical commercial assays |
|
| Picopure™ RNA isolation kit |
ThermoFisher Scientific |
cat # KIT0204 |
| RNeasy minikit |
Qiagen |
cat # 74104 |
| SMART-seq v4 Ultra Low Input RNA Kit for Sequencing |
Takara Bio |
cat # 634888 |
| Nextera XT Library Preparation Kit |
Illumina |
cat # FC-131-1024 |
| NEB Next Ultra Pure RNA library kit for Illumina |
New England Biolabs |
cat #E7770S |
|
| Deposited data |
|
| GSE99115 |
Gene Expression Omnibus |
https://www.ncbi.nlm.nih.gov/geo/
|
|
| Experimental models: Cell lines |
|
| HASTR/ci35 |
Furihata, T. et al.54
|
N/A |
|
| Experimental models: Organisms/strains |
|
| Tg(tetO-HIST1H2BJ/GFP)47Efu/J × Tg(Fos105 tTA)1Mmay |
Matsuo, N. et al.33
|
RRID:IMSR_JAX:005104 |
| S1P1
flox/flox:hGFAP-cre mice |
Choi, J.W. et al.29
|
N/A |
| CD320-KO |
Lai, S.C. et al.43
|
N/A |
|
| Oligonucleotides |
|
| PCR primer sets |
This paper |
Table S4
|
|
| Software and algorithms |
|
| FlowJo (10.0.8r1) |
BD |
N/A |
| Prism |
GraphPad |
N/A |
| R |
r-project.org
|
RRID:SCR_001905 |
|
| Other |
|
| Vitamin B12-deficient diet |
Research Diets, Inc |
N/A |
